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Precision was 20% at LLOQ (0.01%). The limit of blank was 10/500,000 cells. Concordance between the 14-color and legacy assay (Deming regression y = 1.01x, r = .99) was seen. All 20 samples with MRD levels 0.5%-0.006% (median 0.04%) showed an abnormal cell cluster by bh-SNE, with concordant results between manual and automated quantitation (y = x, r = 1). CLL cases clustered together and away from mantle cell lymphoma by bh-SNE and PCA with outlier atypical phenotype CLL cases posing diagnostic challenges by both manual and automat

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