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As the control IT showed optimal usage, with an RSY worth of 11.12% in the day of hatch, the cool and hot IT groups exhibited reduced application with an RSY value of 18.18 and 29.99%, respectively. These findings will be the very first to exhibit that ITs change the expression of crucial YST genes, leading to variations in yolk usage by the embryo.A 3 × 2 factorial arrangement of remedies was performed to investigate the effects of metal (Fe, 40, 60, and 80 mg/kg) and Bacillus subtili
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