https://www.selleckchem.com/pr....oducts/rucaparib.htm
Here we provide a detailed protocol for the simple and rapid NGS of rAAV genomes from DNA extracts. With this protocol users can quickly confirm the identity of an rAAV preparation and detect the presence of contaminating rAAV DNA. Additionally, we share custom Python scripts that allow users to accurately determine the serotype and detect Cre-independent DNA recombination events in rAAV containing Lox sites within minutes. We have used these scripts to analyze over 100 rAAV preparations. While we focused on the detection of cross-con