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Background JC polyomavirus (JCPyV) establishes a stable and successful interaction with the host, causing progressive multifocal leukoencephalopathy (PML) in immunocompromised subjects. Recently, it has been reported that JCPyV, like other viruses, may exploit extracellular vesicles (EV) in cell cultures. Objective To investigate the presence of JCPyV-DNA in EV circulating in human plasma obtained from patients at risk for PML. Study design JCPyV-DNA status was studied in EV obtained from 170 plasma samples collected from 120 HIV positiv