https://www.selleckchem.com/pr....oducts/sch-442416.ht
Understanding the most appropriate workflow for biochemical HLA-associated peptide enrichment prior to ligand sequencing is essential to achieve optimal sensitivity in immunopeptidomics workflows. We describe the use of different detergents for HLA solubilisation as well as complementary workflows to separate HLA-bound peptides from HLA protein complex components after their immunoprecipitation including HPLC, C18 cartridge and 5 kDa Filter. We observed that all solubilisation approaches tested led to similar peptide ligand identific
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