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SIGNIFICANCE AND IMPACT OF THE STUDY The quantity and quality of DNA extracted from human faecal samples is a first important step to optimize molecular methods. Here we have shown that the PowerMicrobiome kit is an effective DNA extraction method for bacterial cells in faecal samples for downstream qPCR purpose. © 2020 The Authors. Journal of Applied Microbiology published by John Wiley Sons Ltd on behalf of Society for Applied Microbiology.BACKGROUND Minimally invasive surgery is heavily dependent on indirect visualization and im
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