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Mixing PESA and PASP can gave nucleation retardation of about 19 h, which approximates the 24 h water residence time in industrial cooling circuits, as well as almost 90% calcium carbonate crystal growth inhibition. This synergy offers promising prospects for preventive scaling treatment.Aim of this study was to investigate the impact of advanced wastewater treatment techniques (combining ozonation with activated carbon filtration) on acute and genotoxic activities of tertiary treated wastewater. Concentrated samples were tested in Salmo