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The widespread use of P-nitrophenol (PNP) as a raw material in pesticides, medicines and dyes has led to environmental pollution. PNP is a well-known endocrine disruptor in mammals and quails. This study investigated the effects of long-term PNP exposure on the testicular development and semen quality of roosters. Pubescent and postpubescent animals were given drinking water supplemented with (0 mg/L, 1 mg/L, 10 mg/L, or 100 mg/L) PNP for eight weeks or sixteen weeks. The relative testis weight, antioxidant index, serum hormone concent
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