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Nonetheless, for many cellular subtype-specific studies, it is hard or impossible to acquire such large numbers of cells and measurement of uncommon histone PTMs is often unachievable. An established specific LC-MS/MS technique ended up being utilized to quantify the abundance of histone PTMs from cell outlines and primary real human specimens. Test planning ended up being changed by omitting atomic isolation and decreasing the rounds of histone derivatization to enhance recognition of histone peptides
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