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05). There was a significant difference (P≤0.05) in sperm abnormality of P0; P1 with P2; P3; P4. The abnormality between P0; P1 and P2; P3 showed no significant difference (P≥0.05), while P2; P3 showed a significant difference with P4 (P≤0.05). The best result in sperm quality was supplementation with 1.5mM α-tocopherol. Ca intracellular intensity 142.76± 21.8 au (P and 176.06±61.43 au (P3). It was concluded that 1.5mM α-tocopherol is the best dose to improve sperm quality by regulating intracellular Ca intensity on Simmental bull cattle. I

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