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Photochemical quenching, non-photochemical quenching, photosynthetic electron transport rate, and the effective quantum efficiency were found to be increased significantly (p less then 0.05) in Avi2 + AA as compared to other MS treatments. Moreover, rice roots harbored significantly (p less then 0.05) higher copy number of nifH gene in Avi2 + AA treatment followed by Avi2 compared to flooded control and other MS treatments. Combined application of AA and Avi2 also increased the grain yield significantly (p less then 0.05) by 7.09 % and 3