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The effects of two representative surfactants, Rhamnolipids and Tween 80, on the microbial diversity of a PCE-degrading consortium during surfactant-enhanced biodegradation, were explored. The biodegradation efficiency was increased from 47.25% to 73.44%, and 47.25%-66.69%, with the addition of Rhamnolipid at 10 mg/L and Tween 80 at 50 mg/L, respectively. PCE biodegradation kinetics can be described by the pseudo-first-order reaction model for both scenarios. Analyses of alpha and beta indices of the microbial consortium showed that the