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Completely, our results revealed that eDNA ddPCR signals lagged behind male chorusing by a few days; hence, acoustic monitoring is preferable in the event that desire is to report the onset of male chorusing. Nevertheless, eDNA could be an effective, non-invasive monitoring tool for amphibians that don't phone and may also supply a useful complement to automatic acoustic recording. We found inhibition habits had been heterogeneous across time and space and we illustrate t
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