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evidence to support the efficacy of this procedure for management of soft tissue defects in the medial thigh region. Escherichia coli AFP111 was previously engineered for succinate production by eliminating byproducts of synthesis pathways. Still, the succinate yield is limited due to the insufficient NADH supplement, when fed with glucose. Microbial electrolysis cell (MEC) allows microorganisms to perform unbalanced fermentation by establishing polarized cathode interaction. In this study, a cathode electrode was used as an additional

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