https://www.selleckchem.com/pr....oducts/bgj398-nvp-bg
Pipetting and concentration measurement of viscous ultra-high-molecular-weight (UHMW) DNA samples is challenging and often highly imprecise. Effective guidelines for handling UHMW samples are missing in the field. Herein, a simple and low-cost workflow is presented that enables accurate pipetting and reliable concentration measurement. Central to the workflow is the shearing of representative small aliquots of UHMW DNA samples to a fragment size less then 150 kb by vortexing them for 1 min with a glass bead in a round-bottomed
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