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Titanium alloys are commonly used in orthopedic devices due to their good corrosion resistance, high specific strength and excellent biological response. The direct contact between the implant surface and the host tissue results in notable effect of surface properties such as surface topography on the biological responses. The aim of this study is to investigate the effect of frequency of pulsed Nd-YAG laser on Ti6Al4V alloy surface topography and its influence on the improvement of biocompatibility while other laser parameters kept c