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https://forum.acronis.com/user/750304
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-down assays are a classic method for studying protein-protein interactions. In -down assay, a bait protein (typically a fusion protein) is immobilized on a solid support, such as a resin or bead. containing the potential interacting proteins is then incubated with the bait protein, allowing the interaction partners to bind to it. After the binding step, the complex is washed to remove unbound proteins, and the interacting proteins are eluted and analyzed.
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