https://notes.io/w6vMs
Following 4 washes with an ice-cold buffer (50 mM Tris, pH 7.four, and zero.05% bovine serum albumin), filters were punched out into 7 ml vials into which 4 ml of RPI Bio-Safe II scintillation fluid (RPI Research Products, Mount Prospect, Illinois) was added. Liquid chromatography/mass spectrometry information had been analyzed using Waters MassLynx 4.1 with assistance from the MetaboLynx utility manager. Automated knowledge processing with MetaboLynx was supplemented by guide interrogation of the information using mass defect filtering, precursor ion, and fragme
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