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Semen frozen in microdialysis tubes produced higher motility and sperm viability than semen frozen with the CryoLock device. The same trend was observed between vapor immersion and vitrification, with vapor immersion proving superior. Vapor immersed semen dialyzed with 20% Me2SO produced statistically similar sperm motility (4 ± 0.41) and viability (73.51% ± 5.56%) to slow-programmable freezing (4.25 ± 0.25, 80.61% ± 4.20%) and the non-frozen control (4.5 ± 0.28, 93.39% ± 0.90%). Optimization of the dialysis process and freezing rate ma
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