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In a 1.3-L bioreactor, the utmost CALB activity and total protein content reached 444.46 ± 18.81 U/mL and 5.41 ± 0.1 g/L, correspondingly, after about 9 times of incubation in FM22 medium, that have been 34 times and 20 times higher than the initial strains, correspondingly. In inclusion, the gotten CALB was made use of to catalyze the transesterification of acidified gutter oil with methanol, recommending a promising pathway to convert waste or poor of bio-oil feedstocks with a high le
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