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Also, we examined the DNA yield of samples from a ditch with an all natural M. fossilis populace month-to-month over 12 months to determine the ideal sampling duration. Our outcomes indicated that the long-term liquid preservation technique commonly used for eDNA surveys of M. fossilis would not induce optimal DNA yields, and now we provide a valid long-term test preservation option. A cost-efficient large salt DNA extraction resulted in the highest target DNA yields and wil

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