https://www.selleckchem.com/pr....oducts/bi-1015550.ht
Single amino acid mutations provide quantitative insight into the energetics that underlie the dynamics and folding of membrane proteins. Chemical denaturation is the most widely used assay and yields the change in unfolding free energy (ΔΔG). It has been applied to 80 different residues of bacteriorhodopsin (bR), a model membrane protein. However, such experiments have several key limitations 1) a nonnative lipid environment, 2) a denatured state with significant secondary structure, 3) error introduced by extrapolation to zero
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