https://www.selleckchem.com/pr....oducts/ly2801653-mer
From a mechanistic point of view, we speculated that the imine intermediate, synthesized by oxidases or dehydrogenases, could be converted into primary α-aminonitrile by nucleophilic addition of cyanide in aqueous solutions. Nitriles and some unnatural amino acids were synthesized through a cascade reaction by oxidative cyanation reaction with the variant and a wide substrate specificity nitrilase.Flavin-dependent enzymes catalyze a wide variety of biological reactions that are important for all types of living organisms. K
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