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The simplified ELISA achieved limits of detection (LOD) and quantification (LOQ) of 0.11 ng mL-1 and 0.37 ng mL-1, respectively, in lysed whole blood, and an LOD comparable to that of PCR in Plasmodium in vitro cultures (0.67 and 1.33 parasites μL-1 for ELISA and PCR, respectively). Accordingly, the developed immunoassay represents a simple and effective diagnostic tool for P. falciparum malaria, with a time-to-result of less then 60 min and sensitivity similar to the reference PCR, but easier to implement in low-resource settings.Advances in gas sensing de