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Graphene oxide (GO) and its derivatives are promising metal-free heterogeneous catalysts due to their high surface area and rich chemical properties. We developed a bifunctional boron-doped sulfonated graphene oxide (BS-GO) and demonstrated its excellent catalytic conversion of glucose to 5-hydroxymethylfurfural (HMF) in a one-pot reaction. BS-GO afforded a high HMF yield of 36.0% from glucose without the use of additives or strong acids. Furthermore, the origin of the catalytic active sites of BS-GO was investigated, unveiling the un