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However, use of the 16SNGS platform has both benefits and limitations. In addition, transition from the old-fashioned methods of CBtest to 16SNGS requires procurement of pricey equipment, appropriate and lasting maintenance of those platforms, certain center infrastructure and technical expertise. All those elements pose a challenge for middle-income countries, much more for nations into the reduced middle-income range. In this review, we describe the basis for CBtest and 16SNGS, and discus
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