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Background Gambogenic acid (GNA) is one of the main active components of Gamboge, and its anti-cancer role has been reported in some cancers. The study was to investigate the inhibitory effects of GNA on the proliferation and metastasis of bladder cancer (BC) cells and its potential regulatory mechanisms. Materials and methods BC cell lines (BIU-87 cells, T24 cells and J82 cells) were treated with different doses of GNA for different time, and then the effects of GNA on BC cell were examined in vitro using CCK-8 assay, apoptosis assays