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These changes were accompanied by an increased density of CART-, VAChT-, and nNOS-positive fibres. The results suggest that the enteric nervous system plays an important role in protecting the gastrointestinal tract during acrylamide intoxication.Spacer design in spiral-wound membranes (SWMs) significantly affects the axial pressuredrop in the flow channel but also the deposit layer removal. However, the effects of the spacerdesign and feed flow distribution in the module on the filtration performance have not yet beeninvestigated during the highl