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Correctly, we found that our protospacer adjacent motif (PAM)-free CRISPR/Cas12a-assisted RPA assay, which just averagely impeded RPA at its optimal CRISPR/Cas12a focus, outperformed its counterparts in assay design, sign, sensitivity, and speed. We additionally unearthed that a brand new commercial Cas12a effector may also drive our PAM-free CRISPR/Cas12a-assisted RPA assay and minimize its price, though simultaneously decreasing its signal. Our study while the brand-new
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