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Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.Premise A novel set of microsatellite markers was developed for Juglans sigillata (Juglandaceae), an endemic walnut species in southwestern China, to facilitate cultivar identification and future investigations into the genetic structure and domestication history of this species and its close relatives. Methods and Results We developed 32 microsatellite loci for J. sigillata using genomic data and used them to examine 60