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Optical imaging of zebrafish showed the green fluorescence was well dispersed. We have demonstrated that the CQDs have an excellent biocompatibility and can be used as efficient optical nanoprobes for cell tracking and biomedical labeling except for L929 and PC-3M cells. We have demonstrated that the CQDs have an excellent biocompatibility and can be used as efficient optical nanoprobes for cell tracking and biomedical labeling except for L929 and PC-3M cells. Indocyanine green (ICG) has received considerable interest as a biocompatible or