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Furthermore, we shed light on the signaling pathway engaged by BUT to impact the phosphorylation of VEC. Thus, we used phospho-specific antibodies to gauge the phosphorylation of VEC as a result to your SCFA salt butyrate in human aortic endothelial cells (HAOECs) and performed dextran assays to analyze the permeability associated with EC monolayer. The part of c-Src and SCFA receptors FFAR2 and FFAR3 into the induction of VEC phosphorylation had been examined utilizing inhibitors and ant