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AgNPs primarily accumulate in earth through the spreading of sewage sludge (SS). In this study, the effects of direct publicity to AgNPs or indirect exposure via SS contaminated with AgNPs regarding the earthworm Eisenia fetida and soil microbial communities had been contrasted, through 3 situations providing increasing publicity levels. The effects of Ag speciation had been examined by spiking SS with AgNPs or AgNO3 before application to