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The effect of the substrate to inoculum ratio (SIR) on the anaerobic digestion (AD) of the liquid fractions (LFs) of co-hydrothermally treated mixed biomass (sewage sludge (SS) and the organic fraction of municipal solid waste (MSW)) was evaluated. The impact of SS + MSW-hydrochar was also studied at different hydrochar concentrations (0, 1, 5, 10, 15, 20 and 25 ghydrochar/L), in two of the LFs studied. The SIR had a significant impact on methane yield (YCH4) and organic matter degradation, with low methane production (4-44 NmLCH4/g tCO

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