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The COD, NH4+-N, and TN removal percentages decreased with the drop in temperature. However, the plot aerated at the bottom of the first quarter filtration chamber with I. sibirica exhibited the best average CODCr, NH4+-N and TN removal percentages in both the warm season (83.6%, 82.7% and 76.8%) and the cool season (66.3%, 44.1% and 43.8%). Therefore, this study indicated that the combination of aerating at the bottom of the first quarter filtration chamber and planting with I. sibirica in the HSSF CWs would be a promising way forward

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