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The use of natural diatoms is currently a topic of interest for therapeutic applications due to its facilities, low cost, and biocompatibility. Here, we report the chemical modification of diatoms Aulacoseria genus microalgae-derived biosilica from Guayllabamba - Ecuador decorated with gold nanoparticles by In-situ and Ex-situ methods to study the in vitro gentamicin loading and release properties in simulated body fluid (SBF). Successful decoration of the diatoms and loaded with gentamicin was confirmed using Fourier transform infrared