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Tropomyosin (TM) is a highly conserved protein that considered as the major allergen of crustacean and mollusk species, while, fish-TM also shares high homology with low allergenicity. In this study, the amino acid sequence, B cell epitopes and allergenicity of shrimp (Litopenaeus vannamei), which is widely consumed, were evaluated by using immunoinformatic tools, dot-blot, enzyme-linked immunosorbent assay (ELISA) and mediator release assay. Meanwhile, cross-reactivity of allergic epitopes of fish-TM, shrimp-TM and clam-TM were assessed
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