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05) compared to those growing on tissue culture plastic (TCP). Live/dead staining revealed a rare occurrence of dead cells on aligners. The gene expression level of all inflammatory markers in cells grown on aligners' surfaces was significantly increased (p less then 0.05) compared to cells grown on TCP after two days. Gene expression levels of the proteins involved in barrier function significantly increased (p less then 0.05) on aligners' surfaces after two and seven days of culture. Aligners' material exhibits no cytotoxic effect on or

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