https://www.selleckchem.com/pr....oducts/3-amino-9-eth
25-fold enhancement. The recombinant choxAB was purified to homogeneity using Ni2+ -affinity agarose column with specific activity (0.054 U·mg-1 ), yield (8.1%), and fold purification (11.69). Capillary isoelectric-focusing indicated pI of 8.77 for choxAB. LC-MS/MS confirmed the IBs (62 kDa), with 82.6% of the covered sequence being exclusive to A. baumannii cholesterol oxidase (UniProtKB A0A0E1FG24). The 3D structure of choxAB was predicted using the LOMETS webtool with the cholesterol oxidase template of Streptomyces
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