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To compare statistical differences between groups, the ANOVA test was used. From the perspective of pathological scoring, the 60μg, 90 μg, and 120 μg groups had the highest scores using Image J to treat inflammatory cells. In addition, in the prostate interstitium area treated, it was found that the 90 μg group attained the largest prostate interstitial area as well as the highest degree of swelling. From the results, Image J software is an effective tool in the calculating the surface of the prostatic interstitium and the specific loca
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