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5-40 and 30-300μg.mL-1 using direct Fourier function for TM and SL, respectively. The plots of derivative ratio amplitude and the Fourier function ratio coefficient versus concentration were linear over ranges 2.5-20 and 25-250μg.mL-1 for TM and TL, respectively. Higher sensitivity as indicated by lower values of detection and quantitation limits were obtained using Fourier convoluted spectra (ratio or zero order) compared to derivative methods. All validation aspects per ICH guidelines are included. The proposed methods were also appl
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