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cus for the first time, providing new ideas for effective control of this species. Transcriptome analysis of C. italicus eggs treated at different temperatures provided a theoretical basis for further understanding the adaptation mechanism of C. italicus eggs to low temperature. In addition, four potential RNAi target genes were verified in the eggs of C. italicus for the first time, providing new ideas for effective control of this species. Genome-Wide Association Studies (GWAS) are used to identify genes and alleles that contribute to

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