https://www.selleckchem.com/products/nct-503.html
Although multiple-protease based shotgun proteomics method was shown to improve coverage for phosphosite identification, this traditional pipeline is time-consuming and can be of low reproducibility. Here, we demonstrated a multi-in-one strategy to saturate the phosphosite coverage by combining the multiple-proteases based digestion, one-step enrichment, and one-shot data-independent acquisition (DIA) as short as one hour. In the "three-in-one" workflow, more than 19,700 and 13,500 phosphosites could be identified in the trypsin-like an
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