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02% vs. 2.45%, p0.01). Furthermore, MP induced CD4 T cell apoptosis in vitro, which stimulated HMDM to produce TGFβ. Moreover, elevated TGFβ level in supernatant from HMDM stimulated with MP-treated CD4 T cells promoted Tregs differentiation. MP pulse therapy induces CD4 T cell apoptosis, which promotes monocytes to produce TGFβ and further facilitates Tregs differentiation. Newly-differentiated Tregs suppress proliferation and IFN-γ production of CD4 T cells and contribute to immunoregulatory milieu after MP pulse therapy. MP pulse