https://www.selleckchem.com/pr....oducts/sitravatinib-
Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant library in traditional TIS, the defined mutant library sequencing (DML-Seq) has advantages as 1) efficient mutagenesis; 2) low bottleneck effects; 3) avoid hotpots caused by screening;
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