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Seventy-eight individuals were included 39 with perianal warts and 39 without perianal warts. Subjects with perianal warts more frequently had an abnormal anal cytology (71.8% vs. 38.5%; P=0.003). This group also had a higher rate of anal HPV 16 detection (38.5% vs. 12.8%; P=0.01). No differences were detected in the proportion of patients with anal HR-HPV detection, with an abnormal anal biopsy or with anal HSIL. Perianal warts was an independent risk factor for an abnormal anal cytology (OR 7.2) and for anal HPV 16 detection (OR 6.7)