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This study aimed to globally assess heat strain, dehydration, and mechanical load as acute kidney injury (AKI) indicators in amateur endurance trail athletes during a 35.3 km run. Thirty amateur experienced trail runners completed an endurance trail run (total positive ascend 1815 m). The following assessments were performed at four measurement time points (pre-, during, immediately post [-post ], and after 24 h of the finish of the run [-post ]) serum test (creatinine, blood ureic nitrogen, albumin, creatine kinase, blood ureic nitrog