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TFF-1 expression correlated with the expression of HNF4alpha and MUC5AC (p less then 0.0001, p less then 0.0001, respectively) and inversely correlated with that of TTF-1/NKX2-1 (p less then 0.0001). These results indicate that TFF-1 is characteristically expressed in non-TRU-type adenocarcinomas with gastrointestinal features. TFF-1-positive cases harbored KRAS mutations at a high frequency, but no EGFR or ALK mutations. TFF-1 expression correlated with tumor spread through air space (STAS), and a poor prognosis in advanced stages. Mo
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