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Finally, we further integrated DE and network results to prioritize genes by its functional importance and identified a top-ranked novel gene, LOC107773232, as a potential regulator involved in the carotenoid metabolism pathway. Thus, the results and systems-biology approaches provide a new avenue to understand the molecular mechanisms underlying complex genetic and environmental perturbations in tobacco. © The Author(s) 2020. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.Hepatocellular carcinoma (HCC) is
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