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Genetic variation of two Ziziphus spina-christi L. (Sidr) varieties was determined by Random Amplified Polymorphic DNA (RAPD). The activity of antioxidant enzymes (peroxidase and catalase) was also determined for the two varieties (Balady and Pakistani). Moreover, the antibacterial effects of different concentrations of aqueous and ethanolic extracts of leaves of the two varieties of Ziziphus spina-christi L. were assessed against Gram-positive bacteria (Streptococcus pyogenes and Staphylococcus aureus) and Gram-negative (Klebsiella pneumoniae,